Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis
نویسندگان
چکیده
منابع مشابه
VDAC2 is required for truncated BID-induced mitochondrial apoptosis by recruiting BAK to the mitochondria.
Truncated BID (tBID), a proapoptotic BCL2 family protein, induces BAK/BAX-dependent release of cytochrome c and other mitochondrial intermembrane proteins to the cytosol to induce apoptosis. The voltage-dependent anion channels (VDACs) are the primary gates for solutes across the outer mitochondrial membrane (OMM); however, their role in apoptotic OMM permeabilization remains controversial. Her...
متن کاملStepwise activation of BAX and BAK by tBID, BIM, and PUMA initiates mitochondrial apoptosis.
While activation of BAX/BAK by BH3-only molecules (BH3s) is essential for mitochondrial apoptosis, the underlying mechanisms remain unsettled. Here we demonstrate that BAX undergoes stepwise structural reorganization leading to mitochondrial targeting and homo-oligomerization. The alpha1 helix of BAX keeps the alpha9 helix engaged in the dimerization pocket, rendering BAX as a monomer in cytoso...
متن کاملApoptosis: it's BAK to VDAC.
The voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane (MOM; Mannella & Kinnally, 2008)—also known as mitochondrial porin—has long been implicated in regulating the mitochondrial response to certain cell death stimuli (Galluzzi & Kroemer, 2007). This includes a potential role in MOM permeabilization (MOMP) during apoptosis, which is a necessary step in the release of cyt...
متن کاملThe mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release.
Cytochrome c release from mitochondria is a key event in apoptosis signaling that is regulated by Bcl-2 family proteins. Cleavage of the BH3-only protein Bid by multiple proteases leads to the formation of truncated Bid (tBid), which, in turn, promotes the oligomerization/insertion of Bax into the mitochondrial outer membrane and the resultant release of proteins residing in the intermembrane s...
متن کاملTyrosine dephosphorylation is required for Bak activation in apoptosis.
Activation of the cell-death mediator Bak commits a cell to mitochondrial apoptosis. The initial steps that govern Bak activation are poorly understood. To further clarify these pivotal events, we have investigated whether post-translational modifications of Bak impinge on its activation potential. In this study, we report that on apoptotic stimulation Bak undergoes dephosphorylation at tyrosin...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2015
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1510574112